Enzymes and Inhibitors
New England Biolabs, Inc. NEBuffer Set (EcoRI, DpnII) – 1.25 ml of each
New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Set contains two 1.25ml vials of each unique NEBuffer for the enzymes EcoRI and DpnII. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer. 1X NEBuffer EcoRI 100 mM Tris-HCl 50 mM NaCl 10 mM MgCl2 0.025% Triton X-100 (pH 7.5 @ 25C) 1X NEBuffer DpnII 50 mM Bis-Tris-HCl 100 mM NaCl 10 mM MgCl2 1 mM DTT (pH 6 @ 25C)
New England Biolabs, Inc. EnGen Lba Cas12a (Cpf1) – 70 pmol
EnGen Lba Cas12a from Lachnospiraceae bacterium ND2006 is a site-specific DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) . Targeting requires a gRNA complementary to the target site as well as a 5' TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a occurs ~18 bases 3' of the PAM and leaves 5' overhanging ends. EnGen Lba Cas12a has Simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N and C-termini of the protein.
Discovery Life Sciences HUMAN FMO1 0.5ML@5MG/ML
Human FMO1 SUPERSOMES;Available size: 0.5 ml. Total protein concentration: 5.0 mg/ml. Catalytic assay: methyl p-tolyl sulfide oxidase activity, representative catalytic activity: 10,000 pmol product/(min x mg protein).
Takara Bio RECOMBINA DNASE I (RNASE-FREE)
Enzyme, Modifying; Recombinant DNase I (RNase-free); Takara; 5000U; 5U/uL; Source: Expressed in non-animal host carrying plasmid encodes the gene of bovine pancreas DNase I; Storage: -20 deg.C; Buffers included
Takara Bio MICROCOCCAL NUCLEASE 15000U
Enzyme; Micrococcal nuclease; 15,000U; Digests single-stranded DNA; Will also digest double-stranded DNA or RNA; Yield 3'-phosphate mononucleotides and oglionucleotides; Requires calcium; Completely inactivated by EDTA and EGTA
Amsbio LLC Heparinase I from Recombinant Flavobacterium heparinum - Research Grade
Heparinase I from Recombinant Flavobacterium heparinum - Research Grade
Amsbio LLC Chondroitinase B from Recombinant Flavobacterium heparinum - Research Grade
Chondroitinase B from Recombinant Flavobacterium heparinum - Research Grade
INTACT GENOMICS INC T4 DNA Ligase 400,000 Units (2000 units/µl)
Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzyme joins DNA fragments with either cohesive or blunt termini and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).Applications• Cloning of restriction enzyme generated DNA fragments• Cloning of PCR products• Next-gen library preparation• Joining linkers and adapters to cohesive or blunt-ended DNA• Nick repair in duplex DNA, RNA or DNA/RNA hybrids• Self-circularization of linear DNA
INTACT GENOMICS INC Endonuclease IV (Nfo) 5000 Units (10 units/µl)
Endonuclease IV (Nfo) from Escherichia coli is a 32-kD metalloprotein that aids in the repair of damaged DNA. The enzyme functions both as an apurinic/apyrimidinic nuclease (1) and as a 3′-terminal di-esterase (1-4). Its 3′-terminal di-esterase activity is important in the repair of DNA strand breaks generated by oxidation and ionic radiation (2, 3). In such events, the strand breaks terminate with either a 3′ phosphate or a deoxyribose fragment, preventing repair by DNA polymerase I or DNA ligase. Endonuclease IV removes the blocking groups, leaving a free 3′-hydroxyl terminus. This enzyme does not have detectable associated exonuclease or DNA N-glycosylase activity (1).Applications• Single cell gel electrophoresis (Comet assay) (5, 6)• Alkaline elution (7)• Alkaline unwinding (8)Product SourceE. coli BL21 (DE3) strain expressing E. coli Endonuclease IV gene.Product Includes• Endonuclease IV (Nfo)• 10x Endonuclease IV reaction buffer
Bioworld IPTG | 367-93-1 | MFCD00063273 | 1g
IPTG | Purity: 98% | Mol Wt: 238.31 | 367-93-1 | MFCD00063273 | 1g
Bioworld IPTG, 50 g
IPTG Isopropyl-β-D-Thiogalactopyranoside. An inducer of β-galactosidase used to promote the expression of proteins in cells controlled by the lac operator system. For blue/white screening, 0.1mM final IPTG concentration in LB (Luria Broth) media is recommended. Techncial Information:Biotechnology Grade.Dioxane free.
Bioworld DNase I (Bovine Pancreas), 50 KU
DNase I DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA in the presence of divalent cations, producing 3'-OH oligonucleotides. Mg+2 in the reaction solution causes the enzyme to produce nicks in double-stranded DNA, while in the presence on Mn+2, DNase I cleaves both strands of the DNA. DNase I is useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA. It is also used in purifying RNA from cells or transcription mixtures. Specifications:CAS: 9003-98-9EC: 232-667-0Source: Bovine pancreasActivity: ≥10000 U/mg.Ultra Pure GradeOur DNase I is guaranteed free of RNase contaminants.
![Bioworld Cellulase RS [Onozuka RS], 5 g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50198965.jpg-250.jpg)
Bioworld Cellulase RS [Onozuka RS], 5 g
From mutant Trichoderma viride derived from the parent strain for Cellulase Onozuka R-10. Cellulase RS contains a high activity of decomposing natural celluloses. Can be used to obtain protoplasts and dissolve cell walls in a wide range of plants.* Soluble in water* Optimum pH range 4.0-5.0* Optimum temperature range 50° to 60°CUsually ships within 24 hours.This product is sold for laboratory research use only.